10x v3 kit. Introduction Chromium Single Cell 3ʹ Reagent Kits v3 Chromium Accessories Recommended Thermal Cyclers Additional Kits, Reagents & Equipment Protocol Steps & Timing Stepwise Objectives Cell 10X Chromium Universal 3’ /5’ Gene Expression Profiling of gene expression levels at single-cell resolution. Stepwise Objectives The Chromium Single Cell Gene Expression Solution with Feature Barcoding technology upgrades short read sequencers to deliver a scalable microfluidic platform for assessing Chromium Single Cell 3' Reagent Kits User Guide (v3. The original v1 chemistry was published by Zheng et al. 1 MiSeq Reagent Kits v3 enable the highest output of all MiSeq kits. Versatile assays are part of end-to Introduction Chromium Next GEM Single Cell 3’ LT Reagent Kits v3. 1 (Dual Index) 10x Genomics Accessories Recommended Thermal Cyclers Additional Kits, Reagents & Equipment Protocol Steps Successful execution of this workflow requires third-party reagents, kits, and equipment in addition to those provided by 10x Genomics. This document The advanced GEM-X technology powering our next generation of Chromium Single Cell Gene Expression (3’ v4) and Single Cell Immune Profiling Support home Universal 3' Gene Expression Documentation Library Construction Chromium Next GEM Single Cell 3ʹ LT Reagent Kits (v3. 1. nded for the Chromium Single Cell 3ʹ v3 protocols. There are multiple versions of the kit based on slightly different chemistries. If you're unsure what you need, use the experiment Fisher Scientific Baltics UAB and 10x Genomics, Inc. The purchase of this product conveys to the purchaser the limited, non-transferable right to use the product in RNA sequencing applications to: (i) Revision Date CG000388 Chromium Next GEM Single Cell 3’ Reagent Kits v3. FOR USE WITH Chromium Next GEM Single Cell 3ʹ Kit v3. 0. 1 (Dual Index) with Feature Barcode technology for Cell Multiplexing User Guide Rev A March 2021 Table of Contents Introduction Chromium Single Cell 3ʹ Reagent Kits v3 Chromium Accessories Recommended Thermal Cyclers Additional Kits, Reagents & Equipment Protocol Steps & Timing Stepwise Objectives The 10 Chromium Single Cell 3' Gene Expression kit keeps evolving. 1 Chemistry Dual Index) with Feature Barcoding technology for Cell Surface Protein GEM-X Universal 3' Gene Expression Product Sheet: Enhance discovery with exceptional sensitivity at single cell resolution Stepwise Objectives The Chromium GEM-X Single Cell Gene Expression v4 upgrades short read sequencers to deliver a scalable microfluidic platform for 3' digital gene expression by profiling 500 Document Revision Summary Introduction Chromium GEM-X Single Cell 5' Reagent Kits v3 8 10x Genomics Accessories 11 Third-Party Items 12 Protocol Steps & Timing 13 Stepwise Objectives 15 Step 2 – GEM Generation & Barcoding The Single Cell 3’ Protocol upgrades short read sequencers to deliver a scalable microfluidic platform for 3’ digital gene expression profiling of 1000 – 6000 GEM-X Universal 5' Gene Expression v3 4-plex Reagent Kits with Feature Barcode technology for Cell Surface Protein MiSeq Reagent Kits v3 enable the highest output of all MiSeq kits. You can Upgrading to Firmware 4. 1 Chemistry Dual Index) with Feature Barcoding technology for CRISPR Screening Chromium Next GEM Chip M Single Cell Kit* 80 rxns PN-1000349 | 16 rxns PN-1000371 (*Included with Chromium Next GEM Single Cell 3' HT Kit v3. 1 Dual Index protocol. Stepwise Objectives The Chromium Single Cell Gene Expression Solution upgrades short read sequencers to deliver a scalable microfluidic platform for 3’ digital gene expression by profiling 500 PN-1000242/PN-3000483: Dual Index Kit NT, Set A: CSV | JSON 3’ v3. The v3 kits retain the same pre-filled, ready-to-use reagent cartridges as the v1 and v2 kits, Question: Which reagent kits are compatible with 5’ v3? Answer: To run the GEM-X 5’ v3 assay, new GEM, Gel Bead, Library, Chip Kits (and optionally, Feature Barcode Kit) are required. A pool of ~3,500,000 This document lists additional equipment, kits, and reagents for the Chromium GEM-X Single Cell and Immune Profiling User Guide protocols. The Single Cell 3ʹ v3 reagents and workflow updates provide enhanced sensitivity, enabling detection of even more unique transcripts per 1 17014382 Additional Kits, Reagents & Equipment The items in the table below have been validated by 10x Genomics and are highly recomm. 1, 4 rxns PN-1000325 Chromium Next GEM Chip L Single Cell Kit, 16 rxns PN-1000321 Dual Index Kit TT Set A, 96 rxns PN-1000215 Species agnostic 3' poly(A) capture-based spatial gene expression assay for expanded whole transcriptome discovery applications. If you're unsure what you need, use the experiment Stepwise Objectives The Chromium Single Cell Gene Expression Solution upgrades short read sequencers to deliver a scalable microfluidic platform for 3ʹ digital gene expression by profiling 500 Introduction Chromium Single Cell 3ʹ Reagent Kits v3 Chromium Accessories Recommended Thermal Cyclers Additional Kits, Reagents & Equipment Protocol Steps & Timing Stepwise Objectives Cell Compare 10x Genomics products and find the one that's right for your needs. 1 Chromium Accessories Recommended Thermal Cyclers Additional Kits, Reagents & Equipment Protocol Steps & Timing Document Revision Summary Introduction Chromium GEM-X Single Cell 3' Reagent Kits v4 7 10x Genomics Accessories 10 Third-Party Items 10 Protocol Steps & Timing 11 Stepwise Objectives 12 Introduction Chromium Next GEM Single Cell 3’ Reagent Kits 10x Genomics Accessories Recommended Thermal Cyclers Additional Kits, Reagents & Equipment Protocol Steps & Timing User Guide | Chromium Next GEM Single Cell 3' HT Reagent Kits v3. This document provides guidance on Stepwise Objectives The Chromium Single Cell Gene Expression Solution upgrades short read sequencers to deliver a scalable microfluidic platform for 3ʹ digital gene expression by profiling 500 Chromium Next GEM Single Cell 3’ Reagent Kits v3. 1, along with information about data SDS, 1000697_1000701_1000891_Chromium-GEM-X-Single-5-GEM-Kits-v3_Ed2. Substituti. 1 Chemistry Dual Index) with Feature Barcoding technology for Cell Surface Protein and Cell Multiplexing Chromium Single Cell 3' Reagent Kits User Guide (v3. 1,Chromium单细胞基因表达谱解决方案，为几百到几万个细胞的细胞鉴定和基因表达谱提供了一种全面的、可 We deliver powerful, reliable tools that fuel scientific discoveries and drive exponential progress to master biology to advance human health. Recommended Visium Introduction The Chromium Single Cell Gene Expression Solution provides a comprehensive, scalable solution for gene expression profiling of hundreds to tens of thousands of cells. 1 Chemistry Dual Index) with Feature Barcoding technology for Cell Multiplexing Chromium Next GEM Single Cell 3’ Reagent 10x Genomics Accessories Recommended Thermal Cyclers Additional Kits, Reagents & Equipment Protocol Steps & Timing Stepwise Objectives Kits Chromium Single Cell 3' Reagent Kits User Guide (v3. 1 (Dual Index) Table of Contents Introduction Chromium Next GEM Single Cell 3' HT Stepwise Objectives The Chromium Single Cell Gene Expression Solution with Feature Barcode technology for CRISPR screening and cell multiplexing upgrades short read sequencers to deliver a Sequencing-ready libraries are generated and sequenced, and the unique 10x barcodes are used to associate reads back to the individual partitions. 1 Chemistry Dual Index) User Guide, CG000315 CG000315_ChromiumNextGEMSingleCell3-_GeneExpression_v3. 3' CellPlex enables The 10X Genomics Chromium Single Cell 3’ GEM Library and Gel Bead Kit v3 enables simultaneous library preparation of hundreds to thousands of individual cells for 3’ digital gene expression profiling Step 2 – GEM Generation & Barcoding The Single Cell 3’ Protocol upgrades short read sequencers to deliver a scalable microfluidic platform for 3’ digital gene expression profiling of 1000 – 6000 The Chromium Single Cell platform was designed to help make single cell studies accessible and approachable for everyone. Click here for the Chromium™ Controller Firmware Update Procedure. , 2017 in Nature Communications. 1, 16 rxns PN-1000268 Chromium Next GEM Single Cell 3ʹ Kit v3. Please click here for further Introduction Chromium Single Cell 3ʹ Reagent Kits v3 Chromium Accessories Recommended Thermal Cyclers Additional Kits, Reagents & Equipment Protocol Steps & Timing Stepwise Objectives Tips & Stepwise Objectives The Chromium Single Cell Gene Expression Solution with Feature Barcode technology for cell multiplexing upgrades short read sequencers to deliver a scalable microfluidic Stepwise Objectives The Chromium Single Cell Gene Expression Solution with Feature Barcode technology for cell multiplexing upgrades short read sequencers to deliver a scalable microfluidic Unlock the full potential of single-cell technology with the latest innovations in transcriptomic profiling from 10x Genomics. It is discontinued now. Chromium Single Cell 3' Reagent Kits User Guide (v3. Get the broadest set of information including Introduction The Chromium Single Cell Gene Expression Solution provides a scalable microfluidic platform for gene expression profiling of 500-10,000 individual cells per sample. 1, 4 rxns PN-1000269 3’ Feature Barcode Kit, 16 rxns PN-1000262 3ʹ CellPlex Chromium GEM-X Single Cell 5' v3 Gene Expression with Feature Barcoding technology for Cell Surface Protein and CRISPR Screening User Guide Universal Gene Expression offers unparalleled species agnostic versatility with whole transcriptome coverage. 1 Chemistry) with Feature Barcoding technology for Cell Surface Protein Stepwise Objectives The Chromium Single Cell Gene Expression Solution with Feature Barcode technology for CRISPR screening and cell multiplexing upgrades short read sequencers to deliver a Like the 3' Gene Expression kit, there are multiple versions of the 10x Chromium 5' Gene Expression kit based on slightly different chemistry. 1; 16 rxn can also be ordered separately) Introduction Chromium GEM-X Single Cell 5' Reagent Kits v3 10x Genomics Accessories Third-Party Items Protocol Steps & Timing Stepwise Objectives Tips & Best Practices Introduction Chromium Next GEM Single Cell 3’ Reagent Kits 10x Genomics Accessories Recommended Thermal Cyclers Additional Kits, Reagents & Equipment Protocol Steps & Timing The Chromium Single Cell platform was designed to help make single cell studies accessible and approachable for everyone. 1 and 3' HT v3. Chromium Single Cell 3' Reagent Kits User Guide (v3. The This Technical Note highlights sample preparation, reagents, and workflow specifics for Single Cell 3' HT v3. 00 is required before running this assay. 1 (Dual Index) 10x Genomics Accessories Recommended Thermal Cyclers Additional Kits, Reagents & Equipment Protocol Steps & Timing This series of videos will walk you through the Chromium Next GEM Single Cell 3' v3. 10x Genomics assays can also be modified to For use with: GEM-X Universal 3’ Gene Expression v4 4-plex 16 samples PN-1000779 GEM-X OCM 3' Chip Kit v4 4-plex 2 chips PN-1000747 Software support Cloud Analysis Analyze 10x Genomics data quickly and securely in the cloud. pdf 10x 单细胞转录组 3’ 试剂,基于Next GEM技术的Chromium平台实现了大规模的单细胞集成分析。 Chromium单细胞基因表达解决方案数万个单细 Visium enables unbiased molecular profiling of frozen and fixed tissue sections, simple tissue handling, sensitive gene detection, and user-friendly software. Versatile assays are part of end-to Chromium Universal 3' Gene Expression provides single cell transcriptome 3' gene expression alongside the detection of surface protein expression or CRISPR Stepwise Objectives The Chromium Single Cell Gene Expression Solution with Feature Barcoding technology upgrades short read sequencers to deliver a scalable microfluidic platform for assessing Introduction Chromium Next GEM Single Cell 3’ Reagent Kits 10x Genomics Accessories Recommended Thermal Cyclers Additional Kits, Reagents & Equipment Protocol Steps & Timing For use with: Chromium GEM-X Single Cell 3' Kit v4, 16 rxns PN-1000691 | 4 rxns PN-1000686 Chromium GEM-X Single Cell 3' Chip Kit v4, 4 Technical Note - Chromium™ Single Cell 3’ v2 Libraries – Sequencing Metrics for Illumina® Sequencers (v2 Chemistry) This Technical Stepwise Objectives The Chromium Single Cell Gene Expression Solution with Feature Barcode technology upgrades short read sequencers to deliver a scalable microfluidic platform for assessing Technical Note - Assay Scheme and Configuration of Chromium™ Single Cell 3' v2 Libraries This Technical Note presents a detailed description of the assay configuration for GEM-X Universal 5' Gene Expression cDNA Kit v3, Automated, 16 samples Library Construction Kit (optional) REAGENT 1000694 Library Construction Kit C, 16 This series of videos will walk you through the Chromium Next GEM Single Cell 3' v3. 10X Chromium offers whole GEM-X Universal 5' Gene Expression v3 4-plex Reagent Kits with Feature Barcode technology for CRISPR Screening and Cell Surface Protein. , Introduction Chromium Next GEM Single Cell 3’ Reagent 10x Genomics Accessories Recommended Thermal Cyclers Additional Kits, Reagents & Equipment Protocol Steps & Timing Stepwise For use with: GEM-X Universal 5’ Gene Expression v3 4-plex 16 samples PN-1000780 GEM-X OCM 5' Chip Kit v3 4-plex 2 chips PN-1000748 Each experiment requires a chip, index kit, and core reagents plus an optional feature barcoding kit. In this initial video, find out more about the Chromium Next GEM Single Cell 3' kits Select a product to find user guide for your specific 10x Genomics product. In this initial video, find out more about the Chromium 10x 单细胞测序Next GEM 3′ Kit V3. The Chromium GEM FOR USE WITH Chromium Next GEM Single Cell 3ʹ Kit v3. All third-party reagents and consumables should be obtained prior AI summary: Running the GEM-X 3' v4 assay requires new GEM, Gel Bead, Library, and Chip Kits specific to 3' v4; Dual Index Plates are shared with Next GEM assays like 3' v3. The v3 kits There are multiple versions of the kit based on slightly different chemistries. 0 or higher is required; however the most updated version Each experiment requires a chip, index kit, and core reagents plus an optional feature barcoding kit. 1, 4 rxns PN-1000269 3’ Feature Barcode Kit, 16 rxns PN-1000262 3ʹ CellPlex GEM-X Universal 5' Gene Expression v3 4-plex Reagent Kits with Feature Barcode technology for CRISPR Screening Stepwise Objectives The Chromium Single Cell Gene Expression Solution upgrades short read sequencers to deliver a scalable microfluidic platform for 3ʹ digital gene expression by profiling 500 The Chromium Single Cell Gene Expression Solution provides a comprehensive, scalable solution for gene expression profiling of hundreds to GEM-X Universal 3' Gene Expression v4 4-plex Reagent Kits with Feature Barcode technology for Cell Surface Protein Stepwise Objectives The Chromium Single Cell Gene Expression Solution with Feature Barcode technology for cell surface protein and cell multiplexing upgrades short read sequencers to deliver a Introduction Chromium Next GEM Single Cell 3' HT v3. Introduction The 10x Genomics 3’ CellPlex Kit provides a species-agnostic sample multiplexing solution using a set of 12 Feature Barcode oligonucleotides, each conjugated to a lipid. 1 Dual Index) with Feature Barcode technology for Cell Surface 10x Genomics libraries contain P5 and P7 adapters, which can be used for Illumina sequencing. To run the GEM-X 5' OCM chips in the Chromium X instrument, firmware version 2. 1 is a high throughput solution on the Chromium X series instrument for analyzing hundreds of thousands of cells per run, with up to 16 samples in a FOR USE WITH: Chromium Next GEM Single Cell 3ʹ LT Kit v3. 1 Dual Index Cell Multiplexing libraries are made using the following sample Depending on your experimental goals, additional Library Construction Kits (PN-1000190) may be required. The library structure of v1 and v2 chemistries are exactly the Introduction Chromium Next GEM Single Cell 3ʹ Reagent Kits v3.
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